Chromatography

The SIL-20A/AC is using a high resolution metering pump and the direct sample injection principle for sample injection, which means no loss of sample for the injection process. Even with smallest injection volumes the metering pump resolution of 6 nl provides excellent precision and accuracy. The autosampler can be operated via the Communication Bus Module, CBM-20A / Lite (or by SCL-10AVP System Controller in compatibility mode) as well as in stand alone mode. The module SIL-20AC is equipped with a powerful Peltier Sample Cooler which allows for keeping the sample temperature down to 4° C.

SIL-20A/20AC is capable of handling small injection volumes from 0.1 ml up to 100 ml (2 ml Option) and combines fastest injection speed (10 s for a 10 ml injection) with excellent precision, accuracy and linearity. The autosampler can handle various vial types ( 1, 1.5 or 4 ml vials) as well as microtiter plates (96-well, 96-deep-well or 384-well).

Nearly complete elimination of sample carry over is achieved by the following construction details:

• Direct sample injection principle, in which the sample suction line is part of the flow line.
• Special needle coating
• Minimised contact surfaces of thoroughly selected materials.

For very critical samples the optional second rinsing pump allows further reduction of sample carry over.

To control the entire HPLC system using a chromatography data system or web browser the CBM-20Alite interface can be installed into the SIL-20A/AC.