Shim-pack GIS Series

Shim-pack GIS Series

High retentivity, lower column back pressure, high inertness

Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns.
Highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. The Shim-pack GIS series not only reduces your cost of solvents, but relieves your system load as well. A uniform silica surface and stable chemical modification ensure high analysis reproducibility.

Analysis Examples

Food Dyes

Peaks

 1. Tartrazine  7.6 mg/L
 2. Amaranth  3.8 mg/L
 3. Ingigocarmine  7.6 mg/L
 4. New coccine  3.8 mg/L
 5. Sunset Yellow FCF  5.3 mg/L
 6. Naphthol Yellow S  7.6 mg/L
 7. Uranine  3.8 mg/L
 8. Allura Red AC  5.3 mg/L
 9. Ponceau R  7.6 mg/L
 10. Ponceau SX  5.3 mg/L
 11. Orange I  5.3 mg/L
 12. Fast green FCF  3.0 mg/L
 13. Brilliant blue FCF  3.0 mg/L
 14. Ponceau 3R  7.6 mg/L
 15. Erythrosine  5.3 mg/L
 16. Azure Blue VX  3.0 mg/L
 17. Orange II  7.6 mg/L
 18. Acid red  3.0 mg/L

Conditions

 Column  Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D., 4 μm)
 Eluent  A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4)
 B) CH3CN
 A/B = 90/10 - 50 min - 65/35
 Flow Rate  1.0 mL/min
 Detection  UV 270 nm
 Col. Temp.  40 °C
 Injection Vol.  10 μL

Free Amino acids and Organic acids

Conditions

 Column  Shim-pack GIS CN
 (250 mmL. x 4.6 mmI.D., 5 μm)
 Eluent  20 mM KH2PO4 (pH 4.0; H3PO4)
 Flow Rate  1.0 mL/min
 Col. Temp.  40 °C
 Detection  UV 210 nm
 Injection Vol.  5 μL

Specifications

  Shim-pack GIS C18 Shim-pack GIS CN
Bonded Phase Octadecyl Groups Cyanopropyl Group
Analysis Mode Reversed Phase Reversed Phase, Normal Phase
Features

High retentivity, lower column

back pressure, high inertness

Suitable in either reversed

phase or normal phase mode

Particle Size (μm) 2, 3, 4, 5, 10 3, 5
Pore Size (nm) 10 10
Surface Area (m2/g) 450 450
Carbon Loading (%) 15 14
End Cap -
Inertness ▶▶▶▶▷ -
pH Range 2-7.5 2-7.5
 

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